Hotstar hifidelity polymerase kit manual

HotStar HiFidelity Polymerase Kit For highly specific hot-start PCR with minimal optimization QIAGEN offers an unrivalled portfolio of highly specific and sensitive hot-start PCR products for a wide range of applications with minimal optimization required. Figure 1. Highest specificity with HotStarTaq Plus DNA Polymerase. HotStar HiFidelity DNA Polymerase is an enzyme re-reading-start warm amended only to produce a protrusions, allowing direct and simplified cloning UA / TA. The buffer contains Factor SB prevent degradation of primer and template during PCR setup, providing highly sensitive and reliable high-fidelity PCR. iProof™ High-Fidelity PCR Kit 2 units/µl, 25 µl 50U 2 units/µl, µl U For research purposes only Store at °C iProof is a high-fidelity DNA polymerase that offers extreme performance for all PCR applications. Incorporating an exciting new and patented technology, iProof DNA.

The HotStar HiFidelity procedure is straightforward — simply follow the step-by-step, optimized protocol included with the kit. HotStar HiFidelity DNA Polymerase has been chemically modified to temporarily inactivate not only the polymerase activity, but also the 3'–5' exonuclease activity of the enzyme. HotStar HiFidelity Polymerase Kit For highly specific hot-start PCR with minimal optimization QIAGEN offers an unrivalled portfolio of highly specific and sensitive hot-start PCR products for a wide range of applications with minimal optimization required. Figure 1. Highest specificity with HotStarTaq Plus DNA Polymerase. HotStarTaq DNA Polymerase is shipped on dry ice but retains full activity at room temperature (15–25°C) for 2 weeks. HotStarTaq Master Mix Kit is shipped on dry ice but retains full activity at room temperature (15–25°C) for 3 days. HotStarTaq DNA Polymerase and HotStarTaq Master Mix Kit, including buffers and.

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